Lllt Wound Healing

1. Positive clinical trial report around 92% therapeutic effect.

2. LLLT noninvasive irradiation, no any side effect, totally green physical therapy.

3. Certification and patents protection provided with the passing of CE

4. Imported laser diode to ensure the laser output quality .

5. Small size, easy to carry , and can be treatment at anywhere.

6 .Treatment process is convenient, just connect power bank to use, no adjustment.

①Promote tissue repair

In addition to relieving pain, lasers can also promote tissue repair. When we are injured, the body naturally produces substances called "growth factors" to help repair damaged tissue. Semiconductor lasers can accelerate the production and release of growth factors, thereby speeding up wound healing.

②Improve blood circulation

Poor blood circulation is an important cause of pain. The laser pain treatment device can promote blood flow by increasing blood vessel dilation and improving microcirculation, thereby improving local nutrition and oxygen supply and relieving inflammation and swelling.

Laser pain treatment equipment uses monochromatic light energy generated by lasers to stimulate human tissues through specific wavelengths and powers, thereby relieving pain and promoting tissue repair. Its principle is to use the action of laser energy to change the chemical reaction process inside tissue cells and improve cell activity and metabolism, thereby achieving therapeutic effects.

Background

Low-level laser therapy (LLLT) is increasingly utilized in clinical settings to treat various medical conditions, particularly those involving wound healing. This emerging therapy holds promise for enhancing wound recovery and improving clinical outcomes in both human and veterinary medicine. However, a universally accepted theory explaining all cellular and biological effects of LLLT in tissue repair is still lacking. This study aimed to investigate the impact of LLLT on the migration and proliferation of cultured canine epidermal keratinocytes (CPEK) in an in vitro wound healing model.

Methods

The CPEK cells used in this study were cryo-preserved, isolated from normal tissue, and free of any transformation events. These cells were received frozen in 1 mL vials. All cell cultures were maintained in CnT-09 medium, which includes both basal medium (CnT-BM.2, 500 mL) and supplements (A [10% fetal bovine serum (FBS), 50 mL] and B [L-glutamine, 5 mL]). For this experiment, concentrations of 10% and 1% (serum-deprived medium) were used.

Canine epidermal keratinocyte cells were cultured in 10 mL of 10% serum medium (CnT-09) within 75 cm³ tissue culture flasks, incubated at 37 °C with 5% CO2 and 95% air. They were passaged at 50%-90% confluence, with medium changes every forty-eight hours until sufficient cell numbers were obtained. For scratch migration and proliferation assays, cells were grown to approximately 90% and 40% confluency, respectively. All experiments utilized third- to fifth-passage cells.

Results

Keratinocyte migration and proliferation were evaluated using scratch migration and proliferation assays, respectively, with fifteen independent replicates for each assay. Cells exposed to LLLT at doses of 0.1, 0.2, and 1.2 J/cm² demonstrated significantly faster migration (p < 0.03) and higher proliferation rates (p < 0.0001) compared to non-irradiated cells and those exposed to a higher energy dose of 10 J/cm². Exposure to 10 J/cm² resulted in reduced migration and proliferation. These findings indicate a measurable, dose-dependent effect of LLLT on keratinocyte biology in vitro.

Conclusion

In this in vitro wound healing model, LLLT enhanced cellular migration and proliferation at doses of 0.1, 0.2, and 1.2 J/cm², while a dose of 10 J/cm² inhibited these processes. These results suggest that the positive effects of LLLT observed in vivo may be partially attributed to its influence on keratinocyte behavior.